郭淑元，理学博士，现为北京理工大学生命学院教授，博士生导师，北京理工大学研究生院督导组督导专家。

一、联系方式

地址：北京理工大学生命学院

电子邮件：guosy @bit.edu.cn

二、教育背景

2001年9月－2004年7月，清华大学生物科学与技术系，获博士学位；

1992年9月－1995年7月，东北农业大学生物工程系获硕士学位；

1988年9月－1992年7月，东北农业大学生物工程系获学士学位。

三、工作简历

2004年7月-今，历任北京理工大学生命学院副教授、教授；

2008年6-12月，法国农业科学院，客作研究；

2010年9-11月，法国农业科学院，访问学者；

1995年7月-2001年8月，哈尔滨医科大学基础医学学院微生物学教研室历任助教、讲师。

四、社会兼职

       国际无脊椎动物病理学会细菌学分会(the Society for Invertebrate Pathology, SIP, bacteria branch)历任理事、秘书长。

五、研究领域/方向 

       研究方向为基于人工智能的微生物基因组快速编辑方法的建立及优化、微生物与宿主的互作机理、生物活性蛋白的功能与应用开发等。

具体研究内容包括但不限于：

1. 蛋白质的结构、功能及应用研究；

2. 基于人工智能算法，设计及优化基因编辑方法，实现微生物基因组的高效编辑；

3. 利用CRISPR/Cas9等基因编辑技术，构建高效微生物细胞工厂；

4. 探究微生物（芽胞杆菌）与宿主的互作机理。

       参加国家重点研发专项、国家自然科学基金国际合作重点项目、国家科技部973、863等项目研究，主持国家自然基金项目等。

六、代表性文章

1. Yan Xia, Xiaowen Du, Bin Liu, Shuyuan Guo*, Yi-Xin Huo*. Species-specific design of artificial promoters by transfer-learning based generative deep-learning model. Nucleic Acids Research. 2024, 52(11): 6145-6157.

2. Pengyu Dong, Yunjuan Fan, Yi-Xin Huo, Lichao Sun*, Shuyuan Guo*, Pathway-Adapted Biosensor for High-Throughput Screening of O‑Methyltransferase and its Application in Vanillin Synthesis. ACS Synth. Biol. 2024, 13, 2873−2886.

3. Yan Xia, Lichao Sun, Zeyu Liang, Yingjie Guo, Jing Li, Dan Tang, Yi-Xin Huo*, Shuyuan Guo*. The construction of a PAM-less base editing toolbox in Bacillus subtilis and its application in metabolic engineering. Chemical Engineering Journal, 2023, 469, 143865.

4. Ying Liu, Lichao Sun*, Yi-Xin Huo, Shuyuan Guo*. Strategies for improving the production of bio-based vanillin. Microbial Cell Factories, 2023, 22: 147.

5. Changgeng Xu, Qin Zou, Jiheng Tian, Mengyuan Li, Baowen Xing, Julia Gong, Jiangyun Wang*, Yi-Xin Huo*, Shuyuan Guo*. Simplified Construction of Engineered Bacillus subtilis Host for Improved Expression of Proteins Harboring Noncanonical Amino Acids. ACS Synthetic Biology, 2023, 12(2):583-595.

6. Zeyu Liang, Chaoyong Huang, Yan Xia, Zhaojin Ye, Shunhua Fan, Junwei Zeng, Shuyuan Guo, Xiaoyan Ma, Lichao Sun, Yi-Xin Huo*. Identification of functional sgRNA mutants lacking canonical secondary structure using high-throughput FACS screening. Cell Reports. 2024, 43(6):114290.

7. Jiheng Tian, Baowen Xing, Mengyuan Li, Changgeng Xu, Yi-Xin Huo*, Shuyuan Guo*. Efficient Large-Scale and Scarless Genome Engineering Enables the Construction and Screening of Bacillus subtilis Biofuel Overproducers. International Journal of Molecular Sciences, 2022, 23, 4853.

8. Mengyuan Li, Yi-Xin Huo*, Shuyuan Guo*. CRISPR-Mediated Base Editing: From Precise Point Mutation to Genome-Wide Engineering in Nonmodel Microbes. Biology, 2022, 11, 571.

9. Tong Wu, Zhenya Chen, Shuyuan Guo, Cuiying Zhang, Yixin Huo*. Engineering transcription factor BmoR mutants for constructing multifunctional alcohol biosensors. ACS Synthetic Biology, 2022, 11(3): 1251-1260.

10. Yongjing Zhang, Aijing Zhang, Mengyuan Li, Kanglai He, Shuyuan Guo*. Nanoparticle-loaded microcapsules providing effective UV protection for Cry1Ac. Journal of Microencapsulation, 2021, 38 (7-8): 522-532.

11. Jiaxin Qin, Zongxing Tong, Yiling Zhan, Christophe Buisson, Fuping Song, Kanglai He, Christina Nielsen-LeRoux*, Shuyuan Guo*. A Bacillus thuringiensis chitin-binding protein is involved in insect peritrophic matrix adhesion and takes part in the infection process. Toxins, 2020, 12(4): 252.

12. Xiaolin He, Zhongqin Sun, Kanglai He*, Shuyuan Guo*. Biopolymer microencapsulations of Bacillus thuringiensis crystal preparations for increased stability and resistance to environmental stress. Applied Microbiology and Biotechnology, 2017, 101:2779–2789.

13. Feng Li, Yue Yan, Dandan Wang, Jie Zhang, Shuyuan Guo*. Cry8Ca2-containing layer-by-layer microcapsules for the pH-controlled release of crystal protein. Journal of Microencapsulation, 2014, 31 (6): 567-572.

14. Wenhui Yang, Kanglai He, Jie Zhang, Shuyuan Guo*. pH-Controlled Bacillus thuringiensis Cry1Ac Protoxin Loading and Release from Polyelectrolyte Microcapsules，PLOS ONE, 2012, 7(9), e45233.

15. Shuyuan Guo, Jie Li, Yanfeng Liu, Fuping Song, Jie Zhang. The role of DNA binding with the Cry8Ea1 toxin of Bacillus thuringiensis. FEMS Microbiology Letters, 2011, 317: 203-210.

16. Shuyuan Guo, Chunlu Zhang, Xiaoyin Lin, Yanrui Zhang, Kanglai He, Fuping Song, Jie Zhang. Purification of an active fragment of Cry1Ie toxin from Bacillus thuringiensis, Protein Expression & Purification, 2011, 78(2): 204-208.

17. Xiaoai Wu, Sheng Ye, Shuyuan Guo, Wupeng Yan, Mark Bartlam, Zihe Rao*. Structural basis for a reciprocating mechanism of negative cooperativity in dimeric phosphagen kinase activity. Federation of American Societies for Experimental Biology Journal (FASEB J.), 2010, 24(1): 242-252.

18. Shuyuan Guo^†, Sheng Ye^†, Yanfeng Liu, Lei Wei, Jing Xue, Hongfu Wu, Fuping Song, Jie Zhang, Xiaoai Wu, Dafang Huang*, Zihe Rao*. Crystal Structure of Bacillus thuringiensis Cry8Ea1: An Insecticidal Toxin Toxic to Underground Pests, the Larvae of Holotrichia parallela, Journal of Structure Biology, 2009, 168: 259-266.

19. Shuyuan Guo, Yancai Zhang, Fuping Song, Jie Zhang, Dafang Huang*. Protease-resistant core form of Bacillus thuringiensis Cry1Ie: monomer and oligomer formed in solution. Biotechnology Letter, 2009, 31: 1769-1774.

20. Shuyuan Guo, Zheng Wang, Shaowei Ni, Xicheng Wang*. Consequences of a six residual deletion from the N-terminal of rabbit muscle creatine kinase, Biochimie, 2003, 29 (10): 999-1005.

21. Shuyuan Guo, Zhi Guo, Qin Guo, Baoyu Chen, Xicheng Wang*. Expression, purification and characterization of arginine kinase from the sea cucumber Stichopus japonicus. Protein Express. Purif., 2003, 29: 230-234.

七、授权专利

1. 郭淑元, 张永静, 张爱晶. 具有抗紫外能力的苏云金芽胞杆菌微胶囊及其制备方法, 专利号: ZL201910586384.2.

2. 郭淑元, 张爱晶, 李建德. 一种苏云金芽胞杆菌菌体微胶囊及其制备方法与应用, 专利号: ZL201811560015.8.

3. 郭淑元, 贺晓琳. 一种微胶囊及其制备方法, 专利号: ZL 201510398884.5.

4. 郭淑元, 李峰, 杨文慧. 苏云金芽胞杆菌原毒素微胶囊及其制备方法, 专利号: ZL201310287081.3.

5. 郭淑元, 杨文慧, 何康来, 张杰, 宋福平. 一种空微胶囊、可酸性或碱性控释的微胶囊及其制备方法, 专利号: ZL201210257659.6.